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Effects of EBNE on the proliferation of B-cell. Notes: CFSE-labeled splenocytes were incubated with 5 μg/mL LPS or EBNE at varying concentrations for 72 hours at 37°C with 5% CO 2 , then stained with CD19 for 30 minutes at 37°C in darkness. The cells were washed twice with PBS, and cell proliferation was measured by flow cytometry and analyzed with FlowJo Version 7.6.5 analysis software. Abbreviations: EBNE, EBN extract; EBN, Edible bird’s nest; CFSE, carboxyfluorescein diacetate succinimidyl ester; CTRL, control; LPS, lipopolysaccharide; EBNL, EBN low dose; EBNM, EBN medium dose; EBNH, EBN high dose; CFDA-SE(PE), carboxyfluorescein diacetate-succinimidyl ester (phycoerythrin); PBS, phosphate buffered saline.
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Effects of EBNE on the proliferation of B-cell. Notes: CFSE-labeled splenocytes were incubated with 5 μg/mL LPS or EBNE at varying concentrations for 72 hours at 37°C with 5% CO 2 , then stained with CD19 for 30 minutes at 37°C in darkness. The cells were washed twice with PBS, and cell proliferation was measured by flow cytometry and analyzed with FlowJo Version 7.6.5 analysis software. Abbreviations: EBNE, EBN extract; EBN, Edible bird’s nest; CFSE, carboxyfluorescein diacetate succinimidyl ester; CTRL, control; LPS, lipopolysaccharide; EBNL, EBN low dose; EBNM, EBN medium dose; EBNH, EBN high dose; CFDA-SE(PE), carboxyfluorescein diacetate-succinimidyl ester (phycoerythrin); PBS, phosphate buffered saline.

Journal: Drug Design, Development and Therapy

Article Title: The improvement effects of edible bird’s nest on proliferation and activation of B lymphocyte and its antagonistic effects on immunosuppression induced by cyclophosphamide

doi: 10.2147/DDDT.S88193

Figure Lengend Snippet: Effects of EBNE on the proliferation of B-cell. Notes: CFSE-labeled splenocytes were incubated with 5 μg/mL LPS or EBNE at varying concentrations for 72 hours at 37°C with 5% CO 2 , then stained with CD19 for 30 minutes at 37°C in darkness. The cells were washed twice with PBS, and cell proliferation was measured by flow cytometry and analyzed with FlowJo Version 7.6.5 analysis software. Abbreviations: EBNE, EBN extract; EBN, Edible bird’s nest; CFSE, carboxyfluorescein diacetate succinimidyl ester; CTRL, control; LPS, lipopolysaccharide; EBNL, EBN low dose; EBNM, EBN medium dose; EBNH, EBN high dose; CFDA-SE(PE), carboxyfluorescein diacetate-succinimidyl ester (phycoerythrin); PBS, phosphate buffered saline.

Article Snippet: Data were acquired on a BD FACSCalibur flow cytometer and analyzed with FlowJo Version 7.6.5 analysis software.

Techniques: Labeling, Incubation, Staining, Flow Cytometry, Software

Effects of EBNE on the proliferation of T-cell. Notes: CFSE-labeled splenocytes were incubated with 5 μg/mL ConA or EBNE at varying concentrations for 72 hours at 37°C with 5% CO 2 , then stained with CD3 for 30 minutes at 37°C in darkness. The cells were washed twice with PBS, and the cell proliferation was measured by flow cytometry and analyzed with FlowJo Version 7.6.5 analysis software. Abbreviations: EBNE, EBN extract; EBN, Edible bird’s nest; CFSE, carboxyfluorescein diacetate succinimidyl ester; ConA, concanavalin A; CTRL, control; EBNL, EBN low dose; EBNM, EBN medium dose; EBNH, EBN high dose; CFDA-SE(PE), carboxyfluorescein diacetate-succinimidyl ester (phycoerythrin); PBS, phosphate buffered saline.

Journal: Drug Design, Development and Therapy

Article Title: The improvement effects of edible bird’s nest on proliferation and activation of B lymphocyte and its antagonistic effects on immunosuppression induced by cyclophosphamide

doi: 10.2147/DDDT.S88193

Figure Lengend Snippet: Effects of EBNE on the proliferation of T-cell. Notes: CFSE-labeled splenocytes were incubated with 5 μg/mL ConA or EBNE at varying concentrations for 72 hours at 37°C with 5% CO 2 , then stained with CD3 for 30 minutes at 37°C in darkness. The cells were washed twice with PBS, and the cell proliferation was measured by flow cytometry and analyzed with FlowJo Version 7.6.5 analysis software. Abbreviations: EBNE, EBN extract; EBN, Edible bird’s nest; CFSE, carboxyfluorescein diacetate succinimidyl ester; ConA, concanavalin A; CTRL, control; EBNL, EBN low dose; EBNM, EBN medium dose; EBNH, EBN high dose; CFDA-SE(PE), carboxyfluorescein diacetate-succinimidyl ester (phycoerythrin); PBS, phosphate buffered saline.

Article Snippet: Data were acquired on a BD FACSCalibur flow cytometer and analyzed with FlowJo Version 7.6.5 analysis software.

Techniques: Labeling, Incubation, Staining, Flow Cytometry, Software